human skin fibroblasts  (ATCC)

Journal: Advanced Science

Article Title: Antifouling All‐Polymeric Microneedle Array for Long‐Term Wearable ECG Monitoring

doi: 10.1002/advs.202512430

Figure Lengend Snippet: Antifouling and biocompatibility analysis of the SBMA‐coated MNE. A) Schematic of the workflow on the MNE involving surface modification of the zwitterionic SBMA for antifouling functionality. B) SBMA‐coated MNE demonstrated high hydrophilicity, with a contact angle of 20.65° ± 0.449°. Measurements were performed on five samples, with error bars indicating the standard deviations. C) Wide‐range XPS spectra indicating surface composition at different surface modifications: pristine, PEDOT:Tos‐coated, PDA‐coated, and SBMA‐coated MNEs. D) Cell viability (live/dead) assay and corresponding fluorescence intensity of L929 (mouse fibroblast cell line) and CCD‐986sk (human dermal fibroblast cell line) after 3 d of exposure to SBMA‐coated MNEs. Green fluorescence represents live cells, whereas red indicates dead cells. “ns” denotes no statistically significant difference. Antifouling performance of different surface modifications evaluated by E) bacterial adhesion of E. coli and F) protein adsorption using BSA‐FITC. All measurements were performed on five samples, with error bars indicating the standard deviations.

Article Snippet: Two fibroblast cell lines were used: L929 murine fibroblasts (RRID: CVCL_0462), a standard cell line for cytotoxicity screening, and CCD‐986sk human dermal fibroblasts (RRID: CVCL_2400), which better reflect the microneedle's potential interaction with human skin tissue (Korean Cell Line Bank, Korea).

Techniques: Modification, Live Dead Assay, Fluorescence, Adsorption

Journal: Biomolecules

Article Title: The Role of miRNA167 in Skin Improvement: Insight from Extracellular Vesicles Derived from Rock Samphire ( Crithmum maritimum )

doi: 10.3390/biom15081157

Figure Lengend Snippet: In vitro functional characterization of Cm-callus EVs. ( A ) No significant changes in the viability of human fibroblasts after treatment with Cm-callus EVs. ( B , C ) Decreased average wound size in wounded human fibroblasts dose-dependently after treatment with Cm-callus EVs ( B ) and evaluation of average wound size in [%] ( C ). ( D ) Changes in mRNA expression of MMP1 , COL1A1 , COL1A2 , VEGFA , and TGFB1 genes in human fibroblasts following treatment with Cm-callus EVs. ( E , F ) Changes and recovery in protein expression of COL1A1 in HFF cells after treatment with Cm-callus EVs following UV irradiation ( E ) and evaluation of relative expression of COL1A1 normalized with DAPI ( F ). (green: COL1A1 labeled by Alexa Fluor 488; blue: nucleus labeled by DAPI, Scale bar: 50 μm), * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Human fibroblast cell lines (HFF and CCD-986Sk) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in Dulbecco’s modified Eagle medium (DMEM; Welgene, Gyeongsan, Republic of Korea), supplemented with 10% fetal bovine serum (FBS; Gibco, Waltham, MA, USA) and 1% penicillin–streptomycin solution (P/S; Gibco, Waltham, MA, USA).

Techniques: In Vitro, Functional Assay, Expressing, Irradiation, Labeling

Journal: Biomolecules

Article Title: The Role of miRNA167 in Skin Improvement: Insight from Extracellular Vesicles Derived from Rock Samphire ( Crithmum maritimum )

doi: 10.3390/biom15081157

Figure Lengend Snippet: In vitro functional characterization of miR167. ( A ) No significant changes in the viability of human fibroblasts after transfection with the miR167 mimic. ( B , C ) Decreased average wound size in wounded human fibroblasts in a dose-dependent manner following transfection with the miR167 mimic ( B ) and evaluation of average wound size in [%] ( C ). ( D ) Changes in mRNA expression of MMP1 , COL1A1 , COL1A2 , VEGFA , and TGFB1 genes in human fibroblasts after transfection with the miR167 mimic. ( E , F ) Changes and recovery in the protein expression of COL1A1 in HFF cells by transfection with the miR167 mimic after UV irradiation ( E ) and evaluation of the relative expression of COL1A1 normalized with DAPI ( F ). (green: COL1A1 labeled by Alexa Fluor 488; blue: nucleus labeled by DAPI, Scale bar: 50 μm), * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Human fibroblast cell lines (HFF and CCD-986Sk) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in Dulbecco’s modified Eagle medium (DMEM; Welgene, Gyeongsan, Republic of Korea), supplemented with 10% fetal bovine serum (FBS; Gibco, Waltham, MA, USA) and 1% penicillin–streptomycin solution (P/S; Gibco, Waltham, MA, USA).

Techniques: In Vitro, Functional Assay, Transfection, Expressing, Irradiation, Labeling